Yes, a new drive first has to be initialized, usually in Disk Management, then it needs at least one partition created on it which then needs to be formatted followed by a drive letter assigned. Before Win10 or Win8 the general function when using a NAS drive the letter assigned would be Z: then a second NAS would be Y:, etc. Internal drives would be C:, D:, etc. including ODDs. USB drives would follow those but if keeping a USB drive constantly plugged into the same USB port a letter could be assigned. Changing a USB drive to a different port may cause a change in the previous drive letter, but not always. There's always variables with External drives.